Endocrine Abstracts

Polycystic ovary syndrome (PCOS) is characterized by laboratory and/or clinical features consisting of hyperandrogenism with chronic anovulation. and is currently one of the most common endocrinopaties in women of fertile age. PCOS is associated with a variety of endocrine and metabolic disturbances. It is well known that women are more prone to develop autoimmune diseases compared to men and levels of sexual hormones are thought to be responsible for such a difference. Our ai...

Aim: To investigate total homocysteine (Hcy), cysteine (Cys), glutathione (GSH), lipid and steroid sulfate levels in women with polycystic ovary syndrome (PCOS).Methods: The blood levels of Hcy, Cys, GSH (determined by HPLC), total and HDL-cholesterol, insulin (IRMA),DHEA and DHEAS, cortisol, pregnenolone, 17-hydroxy-pregnenolone and pregnenolone-sulphate (all RIA) were examined in 44 women with PCOS (age 24+/-7yrs) and 12 healthy controls (34+/-5yrs) in...

Aim: To evaluate the effects of cyproteronacetate/ethinylestradiol (Diane 35) on weight, lipid and androgen levels in women with PCOS.Methods: 19 PCOS women, age 24.3 +/- 4.3 yrs, weight (W) 66.3 +/- 13.5 kg. Blood samples in the early follicular phase of menstrual cycle for cholesterol (C), HDL cholesterol (HDL-C), triglycerides (TG), testosterone (T), androstenedione (A), DHEAS and SHBG and again after 7 +/- 3.8 months of treatment with Diane 35.<p...

Aim: To compare influence of transdermal versus peroral estrogen treatment on insulin sensitivity, lipid and hormonal levels in women affected with polycystic ovary syndrome (PCOS).Patients and Methods: 17 women with PCOS, age 24.8 (4.2) years, BMI 24.15 (3.68) were randomly assigned either for peroral (8) or transdermal (9 patients) estrogens in conjunction with cyproteronacetate. In the early follicular phase of menstrual cycle before and then after 3 month of treatment ...

Objective: To investigate a possible association of DRB1, DQA1 and DQB1 alleles with the chronic autoimmune thyroiditis (AT) development in patients with diabetes mellitus type 1 (DM1) manifested between 18 to 40 years of age. Patients and methods: In 118 patients with DM1 (age at DM1 diagnosis 28.0 (6.9) years, duration of DM1 14.0 (6.3) years) HLA-DRB1, -DQA1 and -DQB1 alleles were analysed by sequence specific oligonucleotide probes and sequence specific primers. AT was pre...

The polycystic ovary syndrome (PCOS) is associated with features of the insulin resistance syndrome and altered glucose homeostasis. Factors that play an important role in these processes are still emerging. Adipokines and pro-inflammatory cytokines may be involved in development of insulin resistance in PCOS. The purpose of this study was to determine if a relationship exists between adiponectin, resistin, leptin, interleukin (IL) 4, IL6, IL10, tumor necrosis factor α (T...

Dehydroepiandrosterone (DHEA) and its sulfate derivative (DHEA-S) are major adrenal secretory products in humans, but their biological function is not still fully explained. Some studies reported negative correlation of DHEA(S) with BMI, fat distribution, and correlations with insulin sensitivity or lipid spectra, but the results are often controversial. Our objective was to assess the correlations of DHEA and DHEA-S levels in Czech non-diabetic population with anthropometric,...

SHBG (sex hormone-binding globulin) is a transport protein specific for dihydrotestosterone, testosterone and estradiol. The missense mutation in exon 8 (GAC→AAC) causing the amino acid exchange D327N correlates accordingly to literature data with higher SHBG levels.We studied the possible association of this polymorphism with polycystic ovary syndrome (PCOS) and its influence on anthropometric and biochemical parameters in 247 PCOS patients in com...

The relationship of insulin resistance, androgens and endothelial dysfunction in polycystic ovary syndrome ,PCOS, is controversial till now. We evaluated if ICAM-1 levels differ between PCOS and healthy women and whether this marker of the early endothelial damage is associated with the particular metabolic and/or hormonal characteristics.After signing written informed consent, 39 PCOS women -median of age 25 yrs, BMI 24.9 and 10 age matched healthy wome...

The A-3826G gene polymorphism of the uncoupling protein 1 (UCP1) causes the lower expression of UCP1 in visceral fat and reduced energy expenditure. Peroxisome proliferator activated receptor gamma 2 (PPARG2) plays a role in the regulation of the adipocyte differentiation and energy balance. The Pro12Ala polymorphism is associated with better insulin sensitivity. We compare the genotypic distribution of these polymorphisms in PCOS and controls and study their possible associat...